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Corresponding Author
Djoni Prawira Rahardja

Institutions
Animal Science Faculty - Hasanuddin University, Indonesia

Abstract
The research reported herein is to reveal the effect of in ovo injection (IOI) of L-gln into the eggs of Indonesian native chicken on hatching weight and subsequent weights at 4 and 8 weeks of age. The research was arranged as a factorial experiment 2 x 5 based on a completely randomized design with unequal replication; the first factor was L-gln (L) in isotonic NaCl, with 5 levels: P0 – negative control (without injection), P1 – positive control (isotonic sterilized saline), P2, P3 and P4 – 0.5, 1.0, and 1.5% respectively, and the second was sex (S), with 2 levels (male and female). As much as 97 newly hatched chicks were selected, weighed, tagged the wing, and used in this study. Data were analyzed by variance analysis. The results indicated that from hatching day up to 3 weeks of ages, the weights of newly hatched chicken either with the injection of different levels of L-gln or between the two sexes were not significantly different. There was a strong relationship between egg weight and day old weight of newly hatched chicks, but no significant relationship at further ages. Differences between the two sexes and different levels of L-gln showed a significant effect at 4 weeks of age and continued at 8 weeks. The weights of all IOI chicks were heavier compared to that of the negative control chicks.. The weight of male chicks was heavier compared to that of the female, and there was no significant effect of increasing level of IOI L-gln. The weight of the female chicks increased gradually with increasing level of L-gln. In conclusion, in ovo injection of L-gln positively improved the productivity of Indonesian native chicken in both sexes.

Keywords
Indonesian Native chicken, in ovo injection, L-gln, newly hatched chick weight,

Topic
Animal physiology and behavior

Link: https://ifory.id/abstract/dKycXFPGEumA

Corresponding Author
Djoni Prawira Rahardja

Institutions
Faculty of Animal Science, Hasanuddin University

Abstract
Hatchability and some internal qualities of hatching eggs of Indonesian Native Chicken (INC) were investigated from different egg weights and storage durations. A total of 950 eggs (including 120 eggs for internal quality measurements) were collected and selected from a flock of INC aged 45 – 60 weeks reared in the University Farm, during 3-week production not consecutively. The research was arranged as a 6 x 4 factorial based on a randomized completely block design, using 6 levels of egg storage duration of 1, 2, 3, 4, 5 and 6 days, and 4 levels of egg weight (EW), 3 replication of unequal individual amount in each replication unit. Prior to storing, the eggs in each group were classified according to their weights: (1) ≥35 - <40; (2).≥ 40 -< 45; (3) ≥ 45 - <50 and (4) ≥ 50 - < 55gr. All eggs were sterilized using alcohol 70%, weighed, numbered and stored at room temperature 26-27°C with relative humidity 60-70%. The results indicated that proportion of egg weight loss of lighter egg at different storage duration was mostly significantly higher (P<0.05) than the heavier one. During storage, as the proportion of EW, albumen decreased, yolk and shell slightly increased. Based on correlation coefficient analysis, irrespective of storage duration and egg weight groups, the results indicated that egg weight losses were more closely associated (P<0.05) with alteration in albumen weight than other traits. Hatchability of fertile eggs (%) of all ranges of EW were significantly decreased with longer storage time, and the heavier decreased more significant than that of the lighter one, particularly after 3 d storage. Hatchability of EW more than 40 g showed better at 2 d storage duration compared to that of 1 d storage. Hatchability of EW up to 45 g was significantly better compared to those of heavier EW. In conclusion, hatchability of hatching fertile egg of INC can be maintained at a level higher than 75% up to 3 d storage duration, and then its decrease was markedly for egg weight heavier than 50 g.

Keywords
Indonesian Native Chicken, egg weight, storage duration, internal quality, hatchability

Topic
Animal physiology and behavior

Link: https://ifory.id/abstract/46DtUANLEYyx